negative selection kit Search Results


94
MedChemExpress human cd8 t cells
Human Cd8 T Cells, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MedChemExpress mouse cd8 t cells
Mouse Cd8 T Cells, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MedChemExpress mouse naïve cd4 t cells
Mouse Naïve Cd4 T Cells, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc cd4 negative selection kit
To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate <t>CD4</t> + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.
Cd4 Negative Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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STEMCELL Technologies Inc naive cd4 + t cells
To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate <t>CD4</t> + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.
Naive Cd4 + T Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc negative selection kits
To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate <t>CD4</t> + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.
Negative Selection Kits, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc macs-negative selection kit
To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate <t>CD4</t> + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.
Macs Negative Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc pan-granulocyte negative selection kit
To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate <t>CD4</t> + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.
Pan Granulocyte Negative Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc cd8 magnetic selection kit
To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate <t>CD4</t> + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.
Cd8 Magnetic Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cd4 neg selection kit imag: human cd4 t cell
To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate <t>CD4</t> + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.
Cd4 Neg Selection Kit Imag: Human Cd4 T Cell, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cd3 negative selection kit
To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate <t>CD4</t> + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.
Cd3 Negative Selection Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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STEMCELL Technologies Inc magnetic cd4 + t cell negative selection kit
To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate <t>CD4</t> + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.
Magnetic Cd4 + T Cell Negative Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate CD4 + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.

Journal: bioRxiv

Article Title: Sequence, infectivity and replication kinetics of SARS-CoV-2 isolated from COVID-19 patients in Canada

doi: 10.1101/2020.04.11.037382

Figure Lengend Snippet: To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate CD4 + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.

Article Snippet: PBMCs from healthy donors (n=2; OM8066 and OM8067) were purified into CD4 + , CD8 + , CD19 + , monocytes and others (CD4 − CD8 − CD19 − cells) using CD4 negative selection kit (STEMCELL Technologies, https://www.stemcell.com ), CD8 positive selection kit (STEMCELL Technologies, https://www.stemcell.com ), PE positive selection kit (STEMCELL Technologies, https://www.stemcell.com ) and Monocyte negative selection kit (STEMCELL Technologies, https://www.stemcell.com ), respectively.

Techniques: Infection

To detect coronavirus-like particles in experimentally infected human structural and immune cells, we infected a range of cells with SARS-CoV-2 at a MOI of 0.01 for 48 hours. The cells were fixed, processed and imaged using a transmission electron microscope (n = 10 fields / cell type). Representative image of each cell line is shown above. Virus-like particles are indicated by red arrows. (A) Vero cells. (B) Calu-3 cells. (C) CD4 + PBMC. (D) CD8 + PBMC. (E) CD19 + PBMC. (F) Monocytes from PBMC. (G) Other cells from PBMC (CD4 − CD8 − CD19 − cell populations). (H) THP-1 monocyte. (I) THP-1-derived macrophage. (J) THP-1-derived dendritic cell.

Journal: bioRxiv

Article Title: Sequence, infectivity and replication kinetics of SARS-CoV-2 isolated from COVID-19 patients in Canada

doi: 10.1101/2020.04.11.037382

Figure Lengend Snippet: To detect coronavirus-like particles in experimentally infected human structural and immune cells, we infected a range of cells with SARS-CoV-2 at a MOI of 0.01 for 48 hours. The cells were fixed, processed and imaged using a transmission electron microscope (n = 10 fields / cell type). Representative image of each cell line is shown above. Virus-like particles are indicated by red arrows. (A) Vero cells. (B) Calu-3 cells. (C) CD4 + PBMC. (D) CD8 + PBMC. (E) CD19 + PBMC. (F) Monocytes from PBMC. (G) Other cells from PBMC (CD4 − CD8 − CD19 − cell populations). (H) THP-1 monocyte. (I) THP-1-derived macrophage. (J) THP-1-derived dendritic cell.

Article Snippet: PBMCs from healthy donors (n=2; OM8066 and OM8067) were purified into CD4 + , CD8 + , CD19 + , monocytes and others (CD4 − CD8 − CD19 − cells) using CD4 negative selection kit (STEMCELL Technologies, https://www.stemcell.com ), CD8 positive selection kit (STEMCELL Technologies, https://www.stemcell.com ), PE positive selection kit (STEMCELL Technologies, https://www.stemcell.com ) and Monocyte negative selection kit (STEMCELL Technologies, https://www.stemcell.com ), respectively.

Techniques: Infection, Transmission Assay, Microscopy, Derivative Assay