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Image Search Results
Journal: bioRxiv
Article Title: Sequence, infectivity and replication kinetics of SARS-CoV-2 isolated from COVID-19 patients in Canada
doi: 10.1101/2020.04.11.037382
Figure Lengend Snippet: To determine human cells that support SARS-CoV-2 replication, we infected human cell lines and primary cells at an MOI of 0.01 (n = 2 independent experiments; supernatant from each experiment was titrated in triplicates). We infected Vero E6 cells as a control. THF and Calu-3 cells represent human structural cells. THP-1 is a monocyte cell line that was used to derive macrophages and dendritic cells. PBMCs from healthy human donors (n = 2 independent donors) were used to generate CD4 + , CD8 + , CD19 + , monocytes and other (CD4 − CD8 − CD19 − ) cell populations. Supernatant from infected cells were collected at various times and titrated on Vero E6 cells to determine virus titres (TCID 50 /ml). hpi, hours post infection; CD, cluster of differentiation; PBMC, peripheral blood mononuclear cells.
Article Snippet: PBMCs from healthy donors (n=2; OM8066 and OM8067) were purified into CD4 + , CD8 + , CD19 + , monocytes and others (CD4 − CD8 − CD19 − cells) using
Techniques: Infection
Journal: bioRxiv
Article Title: Sequence, infectivity and replication kinetics of SARS-CoV-2 isolated from COVID-19 patients in Canada
doi: 10.1101/2020.04.11.037382
Figure Lengend Snippet: To detect coronavirus-like particles in experimentally infected human structural and immune cells, we infected a range of cells with SARS-CoV-2 at a MOI of 0.01 for 48 hours. The cells were fixed, processed and imaged using a transmission electron microscope (n = 10 fields / cell type). Representative image of each cell line is shown above. Virus-like particles are indicated by red arrows. (A) Vero cells. (B) Calu-3 cells. (C) CD4 + PBMC. (D) CD8 + PBMC. (E) CD19 + PBMC. (F) Monocytes from PBMC. (G) Other cells from PBMC (CD4 − CD8 − CD19 − cell populations). (H) THP-1 monocyte. (I) THP-1-derived macrophage. (J) THP-1-derived dendritic cell.
Article Snippet: PBMCs from healthy donors (n=2; OM8066 and OM8067) were purified into CD4 + , CD8 + , CD19 + , monocytes and others (CD4 − CD8 − CD19 − cells) using
Techniques: Infection, Transmission Assay, Microscopy, Derivative Assay